1I5Y
HIV-1 GP41 CORE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-12-20 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | H 3 |
| Unit cell lengths | 52.063, 52.063, 59.740 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.990 - 2.100 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1szt |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.029 | 0.142 |
| Number of reflections | 3507 | |
| <I/σ(I)> | 32.2 | 7.3 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | PEG 4000, sodium acetate, ammonium sulphate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
