1I5W
A-DNA DECAMER GCGTA(TLN)ACGC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 5ID-B |
Synchrotron site | APS |
Beamline | 5ID-B |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 1999-03-31 |
Detector | MARRESEARCH |
Wavelength(s) | 0.93217 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 26.140, 43.960, 45.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 17.000 - 1.400 |
Rwork | 0.167 |
R-free | 0.17400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | A-DNA decamer |
RMSD bond length | 0.009 |
RMSD bond angle | 27.460 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.049 | 0.260 |
Number of reflections | 10950 | |
<I/σ(I)> | 38.5 | 6.8 |
Completeness [%] | 99.7 | 96.7 |
Redundancy | 8.6 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6 | 295 | MPD, Spermine, KCl, Sodium Cacodylate, pH 6.0. VAPOR DIFFUSION, HANGING DROP at 295 K, temperature 295.0K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | KCl | ||
2 | 1 | 1 | Sodium Cacodylate | ||
3 | 1 | 1 | Spermine | ||
4 | 1 | 1 | MPD | ||
5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | decamer solution | 2.8 (mM) | |
2 | 1 | drop | MPD | 10 (%) | |
3 | 1 | drop | sodium cacodylate | 40 (mM) | |
4 | 1 | drop | spermine tetrahydrochloride | 12 (mM) | |
5 | 1 | drop | potassium chloride | 80 (mM) | |
6 | 1 | reservoir | MPD | 35 (%(v/v)) |