1I5N
Crystal structure of the P1 domain of CheA from Salmonella typhimurium
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, Hamburg |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-09-18 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9802, 0.9300, 0.9805 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.950, 88.720, 115.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.300 - 2.140 |
R-factor | 0.21 * |
Rwork | 0.210 |
R-free | 0.24800 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.300 | 2.260 |
High resolution limit [Å] | 2.140 | 2.140 |
Rmerge | 0.048 | 0.167 |
Total number of observations | 206627 * | |
Number of reflections | 31247 | |
<I/σ(I)> | 6.8 | |
Completeness [%] | 98.3 | 91.3 |
Redundancy | 6.6 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microdialysis * | 7.5 * | 285 | POLYETHYLENE GLYCOL 2000 MONOMETHYL ETHER, AMMONIUM SULFATE, SODIUM ACETATE, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7 (mg/ml) | |
2 | 1 | reservoir | Tris-HCl | 25 (mM) | |
3 | 1 | reservoir | 25 (mM) | ||
4 | 1 | reservoir | EDTA | 3.5 (mM) | |
5 | 1 | reservoir | beta-mercaptoethanol | 15 (mM) |