1I5I
THE C18S MUTANT OF BOVINE (GAMMA-B)-CRYSTALLIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 296 |
Detector technology | IMAGE PLATE |
Collection date | 1999-06-26 |
Detector | RIGAKU RAXIS II |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 57.850, 57.850, 98.710 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.400 |
Rwork | 0.223 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gcr |
RMSD bond length | 0.012 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.074 | 0.146 |
Number of reflections | 6843 | |
<I/σ(I)> | 21.6 | 9.6 |
Completeness [%] | 97.1 | 92.8 |
Redundancy | 10 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | chill tube containing protein to 265 K, then bring to 295 K | 7 | 295 | 100 mM Phosphate, 20 mM DTT, pH 7.0, chill tube containing protein to 265 K, then bring to 295 K |