1I5E
CRYSTAL STRUCTURE OF BACILLUS CALDOLYTICUS URACIL PHOSPHORIBOSYLTRANSFERASE WITH BOUND UMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 263 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.888 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 89.400, 89.400, 163.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 3.000 |
| R-factor | 0.223 * |
| Rwork | 0.223 |
| R-free | 0.27000 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.425 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.071 | 0.310 |
| Number of reflections | 13156 | 680 * |
| <I/σ(I)> | 21.4 | |
| Completeness [%] | 85.5 | 90.3 |
| Redundancy | 4.8 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG 4000, potassium phosphate, HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | inorganic phosphate | 5 (mM) | |
| 3 | 1 | reservoir | PEG4000 | 6-10 (%) | |
| 4 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
