1I4T

CRYSTAL STRUCTURE ANALYSIS OF RAC1-GMPPNP IN COMPLEX WITH ARFAPTIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2000-06-10
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 41
Unit cell lengths	112.746, 112.746, 68.178
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.600
Rwork	0.259
R-free	0.30400
Structure solution method	MR / MAD
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.099	0.380
Number of reflections	24501
<I/σ(I)>	26.8
Completeness [%]	99.1	94.8
Redundancy	3.2	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	9	18 *	Tris, PEG20K, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	Tris	0.1 (M)
3	1	reservoir	PEG20000	10 (%)