1I4M
Crystal structure of the human prion protein reveals a mechanism for oligomerization
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-09-24 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.0688 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 85.441, 85.707, 40.501 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.000 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.25300 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.170 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.048 | 0.239 |
| Number of reflections | 10069 | |
| <I/σ(I)> | 36 | |
| Completeness [%] | 96.7 | 84.8 |
| Redundancy | 7.8 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 | 20 * | SODIUM CHLORIDE, TRIS HYDROCHLORIDE, CADMIUM CHLORIDE, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8. |
| 3 | 1 | reservoir | 3 (M) | ||
| 4 | 1 | reservoir | 5 (mM) |
