1I4J
CRYSTAL STRUCTURE OF L22 RIBOSOMAL PROTEIN MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.880, 86.590, 38.960 |
| Unit cell angles | 90.00, 104.47, 90.00 |
Refinement procedure
| Resolution | 23.170 - 1.800 |
| R-factor | 0.208 |
| Rwork | 0.209 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1BXE WITH REMOVED BETA-LOOP |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.060 * |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.200 * | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.074 | 0.331 * |
| Total number of observations | 64827 * | |
| Number of reflections | 16162 | 1334 * |
| <I/σ(I)> | 74.7 | 71.3 |
| Completeness [%] | 85.6 | 47.3 |
| Redundancy | 4. * | 3. * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 * | 293 * | Davydova, N., (2001) Acta Crystallogr., Sect.D, 57, 1150. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 50 (mM) | pH5.5 |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | reservoir | 1.9 (M) | ||
| 5 | 1 | reservoir | ethanol | 3 (%) |
