1I4F
CRYSTAL STRUCTURE OF HLA-A*0201/MAGE-A4-PEPTIDE COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-02-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.782 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.467, 80.222, 54.009 |
| Unit cell angles | 90.00, 111.06, 90.00 |
Refinement procedure
| Resolution | 19.900 - 1.400 |
| R-factor | 0.136 * |
| Rwork | 0.136 |
| R-free | 0.17320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hhg |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.090 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 1.440 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.084 * | 0.369 * |
| Number of reflections | 75194 | |
| <I/σ(I)> | 15.2 | 2.3 |
| Completeness [%] | 93.7 | 80.1 |
| Redundancy | 4.3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 291 | used microseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 20 (mM) | |
| 4 | 1 | reservoir | PEG8000 | 23-27 (%) | |
| 5 | 1 | reservoir | Tris-HCl | 100 (mM) |
