1I4A
CRYSTAL STRUCTURE OF PHOSPHORYLATION-MIMICKING MUTANT T6D OF ANNEXIN IV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Spacegroup name | H 3 |
| Unit cell lengths | 119.060, 119.060, 82.160 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.000 - 2.000 |
| Rwork | 0.204 |
| R-free | 0.23000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ann |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.050 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.000 | |
| Rmerge | 0.065 | 0.124 * |
| Number of reflections | 28686 | |
| <I/σ(I)> | 16 | |
| Completeness [%] | 97.9 * | 99.2 * |
| Redundancy | 2 | 1.97 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 17 * | ammonium sulfate, sodium acetate, calcium chloride, pH 5.0, VAPOR DIFFUSION, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | drop | calcium | 20 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 20-35 (%sat) | |
| 4 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 5 | 1 | reservoir | sodium azide | 0.02 (%) | |
| 6 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 7 | 1 | reservoir | 10 (mM) |
