1I3Z

MURINE EAT2 SH2 DOMAIN IN COMPLEX WITH SLAM PHOSPHOPEPTIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2000-01-01
Detector	MARRESEARCH
Wavelength(s)	1.54
Spacegroup name	P 21 21 2
Unit cell lengths	58.994, 59.500, 34.942
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.150
R-factor	0.219
Rwork	0.216
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SAP SH2
RMSD bond length	0.016 *
RMSD bond angle	2.151 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.054	0.330
Total number of observations	24668 *
Number of reflections	7460
<I/σ(I)>	16.6	2
Completeness [%]	97.0	75
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6 *	22 *	0.1 M sodium citrate, 30% PEG 8000, 10mM DTT, 25 mM ammonium sulphate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	PEG8000	30 (%)
3	1	reservoir	sodium citrate	100 (mM)	pH5.6
4	1	reservoir	ammonium sulfate	25 (mM)
5	1	reservoir	dithiothreitol	10 (mM)