1I3W
ACTINOMYCIN D BINDING TO CGATCGATCG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1999-02-26 |
| Detector | CUSTOM-MADE |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 47.013, 47.013, 160.368 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.700 |
| R-factor | 0.231 * |
| R-free | 0.27900 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.024 |
| Data reduction software | d*TREK |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.057 | 0.533 * |
| Number of reflections | 19747 | |
| <I/σ(I)> | 23.3 | 3 |
| Completeness [%] | 95.2 | 88.3 |
| Redundancy | 9.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 4.5 | 4 * | GLYCINE BUFFER, BARIUM CHLORIDE, SPERMINE, PEG400, PH 4.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | glycine buffer | ||
| 2 | 1 | 1 | barium chloride | ||
| 3 | 1 | 1 | spermine | ||
| 4 | 1 | 1 | PEG 400 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA single strand | 1.3 (mM) | |
| 2 | 1 | drop | ActD | 1.3 (mM) | |
| 3 | 1 | drop | glycine buffer | 40 (mM) | |
| 4 | 1 | drop | 10 (mM) | ||
| 5 | 1 | drop | spermine | 3 (mM) | |
| 6 | 1 | drop | PEG400 | 4 (%) | |
| 7 | 1 | reservoir | PEG400 | 50 (%) |
