1I3V
THREE-DIMENSIONAL STRUCTURE OF A LAMA VHH DOMAIN UNLIGANDED
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.669, 56.088, 102.365 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.000 * - 2.030 |
R-factor | 0.211 * |
Rwork | 0.212 |
R-free | 0.24400 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1i3u |
RMSD bond length | 0.015 * |
RMSD bond angle | 2.000 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.120 |
High resolution limit [Å] | 2.010 | 2.010 |
Rmerge | 0.046 * | 0.169 * |
Total number of observations | 57618 * | |
Number of reflections | 16273 | |
<I/σ(I)> | 4 | |
Completeness [%] | 94.6 | 94.6 |
Redundancy | 2.2 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 20 * | PEG MME 5000, Sodium Acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6.7 (mg/ml) | |
2 | 1 | reservoir | PEG5000 MME | 22 (%) | |
3 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.5 |