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1I3V

THREE-DIMENSIONAL STRUCTURE OF A LAMA VHH DOMAIN UNLIGANDED

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU RU200
Temperature [K]298
Detector technologyIMAGE PLATE
DetectorMARRESEARCH
Spacegroup nameP 21 21 21
Unit cell lengths41.669, 56.088, 102.365
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution12.000

*

- 2.030
R-factor0.211

*

Rwork0.212
R-free0.24400

*

Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1i3u
RMSD bond length0.015

*

RMSD bond angle2.000

*

Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareAMoRE
Refinement softwareCNS (1.0)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]25.0002.120
High resolution limit [Å]2.0102.010
Rmerge0.046

*

0.169

*

Total number of observations57618

*

Number of reflections16273
<I/σ(I)>4
Completeness [%]94.694.6
Redundancy2.21.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP5.520

*

PEG MME 5000, Sodium Acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein6.7 (mg/ml)
21reservoirPEG5000 MME22 (%)
31reservoirsodium acetate0.1 (M)pH5.5

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PDB entries from 2024-11-20

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