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1I3Q

RNA POLYMERASE II CRYSTAL FORM I AT 3.1 A RESOLUTION

Replaces:  1EN0
Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRL BEAMLINE BL9-2
Synchrotron siteSSRL
BeamlineBL9-2
Temperature [K]100
Detector technologyCCD
Collection date1999-05-26
DetectorADSC QUANTUM 4
Wavelength(s)1.283
Spacegroup nameI 2 2 2
Unit cell lengths130.700, 224.800, 369.400
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution40.000 - 3.100
R-factor0.229

*

Rwork0.229
R-free0.28300
Structure solution methodMIRAS
RMSD bond length0.008

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RMSD bond angle1.500

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Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareSHARP
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]40.0003.200
High resolution limit [Å]3.1003.100
Rmerge0.0840.298
Number of reflections983159073

*

Completeness [%]99.292.7
Redundancy4.7
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6.5

*

298Fu, J., (1999) Cell, 98, 799.

*

Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11reservoirdioxane50 (mM)
21reservoirdithiothreitol10 (mM)
31reservoirammonium phosphate390 (mM)or sodium phosphate, pH6.5
41reservoirPEG6000
51dropRNA5-8mg

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