1I3P
THE 3.1 ANGSTROM RESOLUTION CRYSTAL STRUCTURE OF A MUTATED BACULOVIRUS P35 AFTER CASPASE CLEAVAGE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 170 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-12-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 75.806, 75.806, 120.693 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.950 - 3.100 |
| R-factor | 0.239 |
| Rwork | 0.239 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p35 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.100 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.054 | 0.395 |
| Total number of observations | 80534 * | |
| Number of reflections | 7577 | |
| <I/σ(I)> | 470.1 | |
| Completeness [%] | 99.0 | 99.6 |
| Redundancy | 10.63 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 25 * | 9-13% PEG 20,000, 100-400 mM NaCl, 100 mM MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 100 (mM) | ||
| 2 | 1 | drop | EDTA | 1 (mM) | |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | 1 (mM) | ||
| 5 | 1 | drop | HEPES | 20 (mM) | |
| 6 | 1 | drop | protein | 5-8 (mg/ml) | |
| 7 | 1 | reservoir | 100-400 (mM) | ||
| 8 | 1 | reservoir | PEG20000 | 9-13 (%) | |
| 9 | 1 | reservoir | MES | 100 (mM) |
