1I3J
CRYSTAL STRUCTURE OF THE DNA-BINDING DOMAIN OF INTRON ENDONUCLEASE I-TEVI WITH ITS SUBSTRATE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-09-20 |
Detector | BRANDEIS - B1 |
Wavelength(s) | 1.2 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.140, 65.210, 43.670 |
Unit cell angles | 90.00, 93.10, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.215 * |
Rwork | 0.215 |
R-free | 0.24800 |
Structure solution method | SIRAS |
RMSD bond length | 0.009 |
RMSD bond angle | 20.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.043 * | 0.389 |
Number of reflections | 17561 | |
<I/σ(I)> | 9 | 3.8 |
Completeness [%] | 99.4 | 96.5 * |
Redundancy | 5.02 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 10 * | PEG3350, MES, glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 10 * | PEG3350, MES, glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 3350 | ||
2 | 1 | 1 | MES | ||
3 | 1 | 1 | glycerol |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4 (mg/ml) | |
2 | 1 | reservoir | PEG3350 | 18 (%) | |
3 | 1 | reservoir | 15 (%) | ||
4 | 1 | reservoir | MES | 0.06 (M) |