1I3G
CRYSTAL STRUCTURE OF AN AMPICILLIN SINGLE CHAIN FV, FORM 1, FREE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 62.200, 62.200, 136.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.440 |
R-factor | 0.175 * |
Rwork | 0.175 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | anti-N9-neuraminidase antibody NC41/anti-lysozyme antibody D11.15 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.811 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | |
High resolution limit [Å] | 2.440 | |
Rmerge | NaN * | |
Total number of observations | 41050 * | |
Number of reflections | 9809 | |
<I/σ(I)> | 11 | |
Completeness [%] | 89.2 | 71.7 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 20 * | Ammonium Sulfate,potassium phosphate, MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 16 (mg/ml) | |
2 | 1 | drop | ammonium sulfate | 1.75 (M) | |
3 | 1 | drop | phosphate | 50 (mM) | pH6.5 |
4 | 1 | reservoir | MPD | 5 (%(v/v)) |