1I2T

X-RAY STRUCTURE OF THE HUMAN HYPERPLASTIC DISCS PROTEIN: AN ORTHOLOG OF THE C-TERMINAL DOMAIN OF POLY(A)-BINDING PROTEIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	103
Detector technology	CCD
Collection date	2000-07-02
Detector	SBC-2
Wavelength(s)	1.100
Spacegroup name	P 21 21 21
Unit cell lengths	32.969, 38.166, 51.062
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.040
R-factor	0.143
Rwork	0.143
R-free	0.17100
Structure solution method	MAD
RMSD bond length	0.011 *
RMSD bond angle	2.060 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	20.000
High resolution limit [Å]	1.040	1.040
Rmerge	0.059	0.300
Total number of observations	460042 *
Number of reflections	31619 *
<I/σ(I)>	38	3
Completeness [%]	99.7	97
Redundancy	13	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.25 *	4 *	Dioxane, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	14 (mg/ml)
2	1	drop		100 (mM)
3	1	drop	HEPES	20 (mM)
4	1	reservoir	1,4-dioxane	34 (%)