1I2L
DEOXYCHORISMATE LYASE FROM ESCHERICHIA COLI WITH INHIBITOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 115 |
| Detector technology | CCD |
| Collection date | 2000-02-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9879 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 39.494, 72.060, 81.662 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.000 - 2.300 |
| Rwork | 0.183 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ADC LYASE |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.088 | 0.217 |
| Number of reflections | 10442 | |
| <I/σ(I)> | 10 | 3.7 |
| Completeness [%] | 95.8 | 91.7 |
| Redundancy | 4.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 20% PEG 4000, 0.2M magnesium acetate, 0.1M HEPES, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
