1I2K
AMINODEOXYCHORISMATE LYASE FROM ESCHERICHIA COLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 115 |
| Detector technology | CCD |
| Collection date | 1999-07-03 |
| Detector | BRUKER |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 40.047, 72.966, 82.871 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.790 |
| R-factor | 0.164 |
| Rwork | 0.164 |
| R-free | 0.24800 |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.024 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 1.900 |
| High resolution limit [Å] | 1.790 | 1.800 |
| Rmerge | 0.089 | 0.362 |
| Number of reflections | 21637 | |
| <I/σ(I)> | 10.7 | 2.8 |
| Completeness [%] | 92.4 | 88.3 |
| Redundancy | 6.8 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 20% PEG 4000, 0.2M magnesium acetate, 0.1M HEPES, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
