1I2H

CRYSTAL STRUCTURE ANALYSIS OF PSD-ZIP45(HOMER1C/VESL-1L)CONSERVED HOMER 1 DOMAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-11-01
Detector	RIGAKU RAXIS V
Wavelength(s)	1.02
Spacegroup name	P 21 21 2
Unit cell lengths	55.262, 71.153, 39.565
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.090 - 1.800
R-factor	0.195 *
Rwork	0.195
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ddw
RMSD bond length	0.020 *
RMSD bond angle	2.168 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	200.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.066	0.268
Total number of observations	50784 *
Number of reflections	14936
<I/σ(I)>	15
Completeness [%]	99.4	96.7
Redundancy	3.4	3.26

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	sodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	23 (mg/ml)
2	1	reservoir	sodium citrate	1.1 (M)	pH6.5