1I2A
CRYSTAL STRUCTURE OF L1 RIBOSOMAL PROTEIN FROM METHANOCOCCUS JANNASCHII WITH 1.85A RESOLUTION.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-12-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.009 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.577, 38.881, 54.936 |
| Unit cell angles | 83.32, 80.14, 74.72 |
Refinement procedure
| Resolution | 19.830 - 1.850 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cjs |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.825 | 1.825 |
| Rmerge | 0.088 | 0.309 |
| Number of reflections | 23155 | |
| <I/σ(I)> | 15 | |
| Completeness [%] | 94.8 | 87.1 |
| Redundancy | 3.52 | 3.41 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 301.5 | PEG 10000, HEPES-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 301.5K |
