1I24
HIGH RESOLUTION CRYSTAL STRUCTURE OF THE WILD-TYPE PROTEIN SQD1, WITH NAD AND UDP-GLUCOSE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-08-23 |
| Detector | CUSTOM-MADE |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 160.243, 160.243, 99.273 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.200 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qrr |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.580 |
| Data reduction software | TRUNCATE |
| Data scaling software | d*TREK |
| Phasing software | CNS (0.9A) |
| Refinement software | CNS (0.9A) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.190 |
| High resolution limit [Å] | 1.140 | 1.140 |
| Rmerge | 0.097 | 0.366 |
| Number of reflections | 196326 | |
| <I/σ(I)> | 21.4 | 1.7 |
| Completeness [%] | 86.4 | 38 |
| Redundancy | 5.9 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | ammonium sulfate, sodium chloride, MES buffer, NAD+, UDP-glucose, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
