1I21
CRYSTAL STRUCTURE OF YEAST GNA1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-09-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9324 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.584, 93.648, 168.311 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 * - 2.400 |
R-factor | 0.221 * |
Rwork | 0.212 |
R-free | 0.28700 * |
Structure solution method | MAD |
RMSD bond length | 0.014 * |
RMSD bond angle | 1.800 * |
Data reduction software | DENZO |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 * | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.078 * | 0.135 * |
Number of reflections | 37568 | |
<I/σ(I)> | 6.3 | 4.8 |
Completeness [%] | 93.8 * | 91.6 * |
Redundancy | 2.2 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | PEG 600, IMIDAZOLE, MALATE, pH 5.10, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG600 | 17-22 (%) | |
2 | 1 | reservoir | imidazole | 0.2 (M) | |
3 | 1 | reservoir | malate | 0.4 (M) | |
4 | 1 | drop | Tris-HCl | 10 (mM) | |
5 | 1 | drop | 150 (mM) | ||
6 | 1 | drop | dithiothreitol | 1 (mM) | |
7 | 1 | drop | protein | 10 (mg/ml) |