1I1Z
MUTANT HUMAN LYSOZYME (Q86D)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Detector | SDMS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.620, 60.820, 33.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.161 |
| Starting model (for MR) | WILD TYPE HUMAN LYSOZYME |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.633 |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 9999.000 * |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.069 |
| Total number of observations | 43723 * |
| Number of reflections | 11872 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6 | 277 | PHOSPHATE, SODIUM CHLORIDE, CALCIUM CHLORIDE, pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | phosphate | 30 (mM) | pH6.0 |
| 2 | 1 | 1 | protein | 20 (mg/ml) | |
| 3 | 1 | 1 | 2.5 (M) | ||
| 4 | 1 | 1 | 1.5 (mM) |
