1I1N
HUMAN PROTEIN L-ISOASPARTATE O-METHYLTRANSFERASE WITH S-ADENOSYL HOMOCYSTEINE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 1997-11-25 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.105, 53.917, 48.814 |
Unit cell angles | 90.00, 116.15, 90.00 |
Refinement procedure
Resolution | 15.000 * - 1.600* |
R-factor | 0.18 * |
Rwork | 0.183 |
R-free | 0.21100 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1DL5.PDB |
RMSD bond length | 0.004 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.550 |
High resolution limit [Å] | 1.600 * | 1.600 * |
Rmerge | 0.039 * | 0.124 * |
Number of reflections | 28488 * | 2587 * |
<I/σ(I)> | 28 | |
Completeness [%] | 95.7 * | 87.1 * |
Redundancy | 1.7 * | 1.4 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 4 * | used macroseeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 5 (%) | |
3 | 1 | reservoir | sodium cacodylate | pH6.5 | |
4 | 1 | reservoir | magnesium acetate | 15 (mM) | |
5 | 1 | reservoir | AdoHcy | 2.6 (mM) |