1I1H
CRYSTAL STRUCTURE ANALYSIS OF PRECORRIN-8X METHYLMUTASE COMPLEX WITH HYDROGENOBYRINIC ACID
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-12-20 |
| Detector | MACSCIENCE |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.100, 36.600, 60.300 |
| Unit cell angles | 90.00, 113.80, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.21800 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.430 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.106 | 0.266 |
| Total number of observations | 17886 * | |
| Number of reflections | 5501 | |
| <I/σ(I)> | 9.3 | |
| Completeness [%] | 98.1 | 98.4 * |
| Redundancy | 3.4 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 299 | PEG 4000, Magnesium Chloride, Tris-HCl, pH 8.50, VAPOR DIFFUSION, HANGING DROP, temperature 299.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 25-32.5 (%) | |
| 3 | 1 | reservoir | 0.2 (M) | ||
| 4 | 1 | reservoir | Tris-HCl | 0.1 (M) |
