1I1G
CRYSTAL STRUCTURE OF THE LRP-LIKE TRANSCRIPTIONAL REGULATOR FROM THE ARCHAEON PYROCOCCUS FURIOSUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-09-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.488 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 101.318, 101.318, 245.361 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| R-factor | 0.316 |
| Rwork | 0.313 |
| R-free | 0.38200 |
| Structure solution method | MIR |
| RMSD bond length | 0.012 * |
| RMSD bond angle | 3.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.970 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.065 | 0.346 |
| Total number of observations | 30436 * | |
| Number of reflections | 13809 | |
| <I/σ(I)> | 14.7 | |
| Completeness [%] | 94.7 | 96.2 * |
| Redundancy | 2.2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.3 * | 290 | Sedelnikova, S.E., (2001) Acta Crystallogr, D57, 886. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 20 (mM) | pH5.3 |
| 3 | 1 | drop | 0.25 (M) | ||
| 4 | 1 | reservoir | ammonium sulfate | 2.4-3.6 (M) | |
| 5 | 1 | reservoir | sodium citrate | 0.1 (M) | pH6.0 |
