1I12
CRYSTAL STRUCTURE OF SACCHAROMYCES CEREVISIAE GNA1 COMPLEXED WITH ACCOA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-11-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9326 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 156.559, 51.842, 92.232 |
| Unit cell angles | 90.00, 107.81, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.300 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.22100 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.045 * | 0.376 * |
| Number of reflections | 547136 | |
| <I/σ(I)> | 7.9724 | 1.9 |
| Completeness [%] | 96.6 * | 92.3 |
| Redundancy | 2.05 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 293 | PEG 600, IMIDAZOLE, MALATE, pH 5.1, VAPOR DIFFUSION, HANGING DROP, temperature 20K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG600 | 17-22 (%) | |
| 2 | 1 | reservoir | imidazole | 0.2 (M) | |
| 3 | 1 | reservoir | malate | 0.4 (M) | |
| 4 | 1 | drop | Tris-HCl | 10 (mM) | |
| 5 | 1 | drop | 150 (mM) | ||
| 6 | 1 | drop | dithiothreitol | 1 (mM) | |
| 7 | 1 | drop | protein | 10 (mg/ml) |
