1I0Z
HUMAN HEART L-LACTATE DEHYDROGENASE H CHAIN, TERNARY COMPLEX WITH NADH AND OXAMATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-10-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.488 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 59.491, 161.683, 137.846 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.179 * |
| Rwork | 0.177 |
| R-free | 0.23000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ldh |
| RMSD bond length | 0.015 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.050 | 0.114 |
| Number of reflections | 37680 | |
| <I/σ(I)> | 21.25 | 8.5 |
| Completeness [%] | 96.0 | 91.1 |
| Redundancy | 3.31 | 3.13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 18 * | drop consists of equal amounts of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | NADH | 2.5 (mM) | |
| 3 | 1 | drop | sodium oxamate | 1 (mM) | |
| 4 | 1 | drop | HEPES | 50 (mM) | |
| 5 | 1 | reservoir | PEG8000 | 21 (%) | |
| 6 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 7 | 1 | reservoir | sodium HEPES | 100 (mM) |
