1I0S

ARCHAEOGLOBUS FULGIDUS FERRIC REDUCTASE COMPLEX WITH NADP+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-09-20
Detector	MARRESEARCH
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	56.047, 56.047, 212.969
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.650
R-factor	0.1915
Rwork	0.191
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i0r
RMSD bond length	0.008
RMSD bond angle	24.570 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.710
High resolution limit [Å]	1.650	1.650
Rmerge	0.054	0.204
Total number of observations	168005 *
Number of reflections	35546
<I/σ(I)>	15.6
Completeness [%]	86.1 *	86.5
Redundancy	4.6	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	4 *	PEG4000, SODIUM ACETATE, TRIS-HCL, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	drop	Tris-HCl	0.1 (M)
3	1	reservoir	PEG4000	21-22 (%(w/v))
4	1	reservoir	sodium acetate	0.1 (M)
5	1	reservoir	Tris-HCl	0.1 (M)