1HZT
CRYSTAL STRUCTURE OF METAL-FREE ISOPENTENYL DIPHOSPHATE:DIMETHYLALLYL DIPHOSPHATE ISOMERASE
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-01-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97858, 0.97873 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 71.400, 71.400, 61.850 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.370 - 1.450 |
R-factor | 0.192 |
Rwork | 0.192 |
R-free | 0.22400 |
Structure solution method | AB INITIO PHASING |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | Oudjama, Y., (2001) Acta Crystallogr., 57, 287. * |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | Oudjama, Y., (2001) Acta Crystallogr., 57, 287. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7 (mg/ml) | |
2 | 1 | reservoir | PEG2000 MME | 10 (%(w/v)) | |
3 | 1 | reservoir | Tris-maleate | 100 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 100 (mM) |