1HZO
STRUCTURE OF CLASS A CEPHALOSPORINASE FROM PROTEUS VULGARIS K1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 1999-05-03 |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.650, 66.150, 134.394 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.000 - 1.750 |
| R-factor | 0.169 * |
| Rwork | 0.169 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bza |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.200 * |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 1.860 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.052 * | 0.200 * |
| Total number of observations | 284238 * | |
| Number of reflections | 49364 | 5658 * |
| <I/σ(I)> | 18.5 | 3.2 |
| Completeness [%] | 92.0 * | 65 * |
| Redundancy | 5.76 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.25 | 293 | PEG 6000, MES, pH 6.25, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | PEG6000 | 12.5 (%(w/v)) | |
| 3 | 1 | drop | MES | 12.5 (mM) | pH6.25 |
| 4 | 1 | reservoir | PEG | 25 (%) | |
| 5 | 1 | reservoir | MES | 25 (mM) |
