1HZI

INTERLEUKIN-4 MUTANT E9A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-06-11
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	91.050, 91.050, 45.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.880 - 2.050
R-factor	0.225
Rwork	0.225
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rcb
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.100
High resolution limit [Å]	2.050	2.050
Rmerge	0.034 *	0.168
Total number of observations	61298 *
Number of reflections	12421	795 *
<I/σ(I)>	34.8	7.7
Completeness [%]	99.2 *	98.8
Redundancy	5	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	293	ammonium sulfate, sodium acetate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium acetate	100 (mM)
2	1	reservoir	ammonium sulfate	63 (%sat)
3	1	drop	protein	10 (mg/ml)