1HZD

CRYSTAL STRUCTURE OF HUMAN AUH PROTEIN, AN RNA-BINDING HOMOLOGUE OF ENOYL-COA HYDRATASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	90
Detector technology	CCD
Collection date	1999-11-17
Detector	MARRESEARCH
Wavelength(s)	1.00
Spacegroup name	P 1 21 1
Unit cell lengths	79.130, 132.070, 80.040
Unit cell angles	90.00, 108.14, 90.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.207 *
Rwork	0.207
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dub
RMSD bond length	0.006
RMSD bond angle	21.302 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.046	0.123 *
Total number of observations	236961 *
Number of reflections	76595
<I/σ(I)>	13.1	2.673
Completeness [%]	96.8	91.5
Redundancy	3.1	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5	293	PEG 8000, calcium acetate, sodium acetate, imidazole, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	sodium cacodylate	50 (mM)	pH6.5
2	1	drop	PEG8000	9 (%)
3	1	drop	calcium acetate	100 (mM)
4	1	drop	sodium acetate	100 (mM)
5	1	drop	imidazole	10 (mM)
6	1	reservoir	sodium cacodylate	100 (mM)	pH6.5
7	1	reservoir	PEG8000	18 (%)
8	1	reservoir	calcium acetate	200 (mM)