1HYR

CRYSTAL STRUCTURE OF HUMAN MICA IN COMPLEX WITH NATURAL KILLER CELL RECEPTOR NKG2D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-10-10
Detector	ADSC QUANTUM 4
Wavelength(s)	1.100
Spacegroup name	P 4 21 2
Unit cell lengths	122.780, 122.780, 101.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.780 - 2.700
R-factor	0.227
Rwork	0.227
R-free	0.28200
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.076	0.425
Total number of observations	256599 *
Number of reflections	21938
<I/σ(I)>	34	4.1
Completeness [%]	99.9	100
Redundancy	11.7	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7 *	22 *	(NH4)2SO4, pH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 295.0K
1	VAPOR DIFFUSION, SITTING DROP	7 *	22 *	(NH4)2SO4, pH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 295.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.5 (mg/ml)
2	1	drop	PIPES	25 (mM)
3	1	drop	EDTA	1 (mM)
4	1	drop		0.02 (%)
5	1	reservoir	ammonium sulfate	0.4 (M)
6	1	reservoir	MES	50 (mM)