1HYQ
MIND BACTERIAL CELL DIVISION REGULATOR FROM A. FULGIDUS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-10-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97900, 0.97920, 0.93930 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 90.312, 90.312, 83.333 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.000 - 2.600 |
R-factor | 0.2058 |
Rwork | 0.206 |
R-free | 0.22520 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.332 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SOLVE |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.071 | 0.281 |
Number of reflections | 10622 | |
<I/σ(I)> | 12.2 | 3.8 |
Completeness [%] | 95.7 | 98.6 |
Redundancy | 3.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.6 | 19 * | 2% PEG6000, 100mM TRIS buffer pH 8.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG6000 | 2 (%) | |
2 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
3 | 1 | drop | protein | 5 (mg/ml) |