1HYH
CRYSTAL STRUCTURE OF L-2-HYDROXYISOCAPROATE DEHYDROGENASE FROM LACTOBACILLUS CONFUSUS AT 2.2 ANGSTROMS RESOLUTION-AN EXAMPLE OF STRONG ASYMMETRY BETWEEN SUBUNITS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-11-30 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 135.900, 135.900, 205.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| R-factor | 0.21 |
| R-free | 0.25000 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.030 |
| Data reduction software | MOSFLM |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.100 |
| High resolution limit [Å] | 2.220 |
| Rmerge | 0.076 |
| Total number of observations | 388074 * |
| Number of reflections | 107769 * |
| Completeness [%] | 94.9 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 19 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | imidazole HCl | 50 (mM) | |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | L-dithiothreitol | 1 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 1.5 (M) | |
| 6 | 1 | reservoir | potassium phosphate | 50 (mM) | |
| 7 | 1 | reservoir | hexantriole | 1 (%(w/w)) | |
| 8 | 1 | reservoir | NAD+ | 30 (mM) |
