1HY2
MINIPROTEIN MP-1 COMPLEX WITH STREPTAVIDIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 278 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-12-17 |
| Detector | MACSCIENCE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.633, 81.364, 83.307 |
| Unit cell angles | 90.00, 105.04, 90.00 |
Refinement procedure
| Resolution | 29.800 - 2.000 |
| Rwork | 0.214 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1SLD STREPTAVIDIN TETRAMER |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.105 | 0.317 |
| Number of reflections | 38522 | |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 97.5 | 88.2 |
| Redundancy | 4.18 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 100mM potassium acetate, ammonium sulfate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
