1HY1
CRYSTAL STRUCTURE OF WILD TYPE DUCK DELTA 2 CRYSTALLIN (EYE LENS PROTEIN)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-07-16 |
| Detector | BRANDEIS - B1 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.481, 99.052, 107.177 |
| Unit cell angles | 90.00, 102.08, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.206 |
| Rwork | 0.208 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 19.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.050 | 0.220 |
| Total number of observations | 277694 * | |
| Number of reflections | 79045 | |
| <I/σ(I)> | 15.2 | |
| Completeness [%] | 93.2 | 92.1 |
| Redundancy | 3.5 | 3.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 14% PEG 2000 MME, 300mM magnesium chloride, 100mM HEPES, pH 7.5. VAPOR DIFFUSION, HANGING DROP at 298 K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | mPEG2000 | 14 (%) | |
| 3 | 1 | reservoir | 300 (mM) | ||
| 4 | 1 | reservoir | HEPES | 100 (mM) |
