1HXZ
MINIPROTEIN MP-2 COMPLEX WITH STREPTAVIDIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-01-14 |
| Detector | MACSCIENCE |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 81.926, 82.599, 89.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.100 - 1.800 |
| Rwork | 0.209 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1SLD STREPTAVIDIN TETRAMER |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | GLRF |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.100 | 1.810 |
| High resolution limit [Å] | 1.800 | 1.780 |
| Rmerge | 0.061 | 0.134 |
| Number of reflections | 28243 | |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 99.2 | 98.7 |
| Redundancy | 4.87 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 100mM potassium acetate, 32% AMMONIUM SULFATE , pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
