1HXX

OMPF PORIN MUTANT Y106F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-01-01
Detector	MACSCIENCE
Wavelength(s)	1.042
Spacegroup name	P 3 2 1
Unit cell lengths	118.105, 118.105, 52.565
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.174 *
Rwork	0.187
R-free	0.24400 *
Structure solution method	difference fourier
RMSD bond length	0.080
RMSD bond angle	0.028
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.200
Rmerge	0.065
Total number of observations	71614 *
Number of reflections	19807
<I/σ(I)>	7
Completeness [%]	97.9 *
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *			Pauptit, R.A., (1991) J. Mol. Biol., 218, 505. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10 (mg/ml)
2	1	2	PEG2000	10.5 (%)