1HXU

OMPF PORIN MUTANT KK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-01-01
Detector	MARRESEARCH
Wavelength(s)	1.54
Spacegroup name	P 3 2 1
Unit cell lengths	119.670, 119.670, 53.490
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 3.000
R-factor	0.2 *
Rwork	0.202
R-free	0.27000 *
Structure solution method	difference fourier
RMSD bond length	0.010 *
RMSD bond angle	0.039 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((AGROVATA)
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	3.000
Rmerge	0.089
Total number of observations	31762 *
Number of reflections	8886
<I/σ(I)>	7.3
Completeness [%]	98.6
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *			Pauptit, R.A., (1991) J. Mol. Biol., 218, 505. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10 (mg/ml)
2	1	2	PEG2000	10.5 (%)