1HXU
OMPF PORIN MUTANT KK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 119.670, 119.670, 53.490 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 3.000 |
| R-factor | 0.2 * |
| Rwork | 0.202 |
| R-free | 0.27000 * |
| Structure solution method | difference fourier |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 0.039 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.089 |
| Total number of observations | 31762 * |
| Number of reflections | 8886 |
| <I/σ(I)> | 7.3 |
| Completeness [%] | 98.6 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | Pauptit, R.A., (1991) J. Mol. Biol., 218, 505. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 10 (mg/ml) | |
| 2 | 1 | 2 | PEG2000 | 10.5 (%) |
