1HXT

OMPF PORIN MUTANT NQAAA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-01-01
Detector	MARRESEARCH
Wavelength(s)	0.992
Spacegroup name	P 3 2 1
Unit cell lengths	117.084, 117.084, 51.344
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.400
R-factor	0.213 *
Rwork	0.233
R-free	0.28500 *
Structure solution method	difference fourier
RMSD bond length	0.010 *
RMSD bond angle	0.033 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.079	0.310
Total number of observations	53590 *
Number of reflections	14853
<I/σ(I)>	7.5
Completeness [%]	93.1	93.5
Redundancy	3.6	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *			Pauptit, R.A., (1991) J. Mol. Biol., 218, 505. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10 (mg/ml)
2	1	2	PEG2000	10.5 (%)