1HXQ

THE STRUCTURE OF NUCLEOTIDYLATED GALACTOSE-1-PHOSPHATE URIDYLYLTRANSFERASE FROM ESCHERICHIA COLI AT 1.86 ANGSTROMS RESOLUTION

Experimental procedure

Temperature [K]	113
Detector technology	AREA DETECTOR
Collection date	1995
Detector	SIEMENS HI-STAR
Spacegroup name	P 21 21 2
Unit cell lengths	57.200, 215.400, 68.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.000 - 1.860
Rwork	0.196
RMSD bond length	0.013
RMSD bond angle	2.146
Data reduction software	SAINT
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.000
High resolution limit [Å]	1.860	1.860 *
Rmerge	0.063	0.165 *
Total number of observations	206202 *
Number of reflections	70581	10989 *
Completeness [%]	97.0	90 *
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6 *		EACH CRYSTAL WAS REACTED WITH SUBSTRATE UDP-GLUCOSE IN A SYNTHETIC MOTHER LIQUOR DEVOID OF LITHIUM SULFATE AT PH 7.1.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG10000	14 (%(w/v))
2	1	reservoir		0.40 (M)
3	1	reservoir		0.25 (M)
4	1	reservoir	sodium succinate	0.10 (M)