1HXL
MINIPROTEIN MP-2 (V10A) COMPLEX WITH STREPTAVIDIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 108 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-01-13 |
| Detector | MACSCIENCE |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 81.989, 82.583, 89.385 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.290 - 1.800 |
| Rwork | 0.215 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.076 | 0.221 |
| Number of reflections | 28454 | |
| <I/σ(I)> | 11.7 | |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 7.86 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 298 | 100 MM POTASSIUM ACETATE, AMMONIUM SULFATE, pH 4.80, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
