1HXK

GOLGI ALPHA-MANNOSIDASE II IN COMPLEX WITH DEOXYMANNOJIRIMICIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2000-05-27
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	68.814, 109.616, 138.281
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	500.000 * - 1.690 *
R-factor	0.1969 *
Rwork	0.197
R-free	0.21560 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hty
RMSD bond length	0.006
RMSD bond angle	1.330 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.900	1.550
High resolution limit [Å]	1.690 *	1.690 *
Rmerge	0.086 *	0.186 *
Number of reflections	114653 *
<I/σ(I)>	18.3	3.3
Completeness [%]	97.8 *	92.6 *
Redundancy	5.8	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7	298	PEG 6000, MPD, Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG6000	8.5 (%)
2	1	reservoir	MPD	5 (%)
3	1	reservoir	Tris	100 (mM)	pH7.0