1HXJ

CRYSTAL STRUCTURE OF THE MAIZE ZM-P60.1 BETA-GLUCOSIDASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-04-26
Detector	MARRESEARCH
Wavelength(s)	0.9831
Spacegroup name	P 1 21 1
Unit cell lengths	55.664, 110.718, 72.940
Unit cell angles	90.00, 92.10, 90.00

Refinement procedure

Resolution	68.000 * - 2.050
Rwork	0.168
R-free	0.23000 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cbg
RMSD bond length	0.005
RMSD bond angle	22.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.000	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.053 *	0.176 *
Number of reflections	52651
<I/σ(I)>	15.5	4.9
Completeness [%]	94.7 *	85.7
Redundancy	9.7	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.6	294	20% PEG 4000, 0.1 M citrate buffer pH 5.6, 0.2 M ammonium acetate, VAPOR DIFFUSION, HANGING DROP at 294K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG4000	20-25 (%(w/v))
2	1	1	citrate	0.1 (M)	pH5.3-5.9
3	1	1	ammonium acetate