1HXI
AN UNEXPECTED EXTENDED CONFORMATION FOR THE THIRD TPR MOTIF OF THE PEROXIN PEX5 FROM TRYPANOSOMA BRUCEI
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-04-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9798, 0.9800, 0.9574 |
| Spacegroup name | P 65 |
| Unit cell lengths | 75.000, 75.000, 56.010 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.191 |
| Rwork | 0.186 |
| R-free | 0.22300 |
| Structure solution method | MAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.079 | 0.502 |
| Number of reflections | 23237 | |
| <I/σ(I)> | 17.3 | |
| Completeness [%] | 98.1 | 99.7 |
| Redundancy | 5.8 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 20 * | Imidazole, MgCl2, IPTG, DMSO, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | imidazole | 100 (mM) | |
| 2 | 1 | reservoir | 50 (mM) | ||
| 3 | 1 | reservoir | IPTG | 5 (mM) | |
| 4 | 1 | reservoir | DMSO | 5 (%(v/v)) |
