1HXI
AN UNEXPECTED EXTENDED CONFORMATION FOR THE THIRD TPR MOTIF OF THE PEROXIN PEX5 FROM TRYPANOSOMA BRUCEI
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-04-17 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9798, 0.9800, 0.9574 |
Spacegroup name | P 65 |
Unit cell lengths | 75.000, 75.000, 56.010 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.600 |
R-factor | 0.191 |
Rwork | 0.186 |
R-free | 0.22300 |
Structure solution method | MAD |
RMSD bond length | 0.020 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.079 | 0.502 |
Number of reflections | 23237 | |
<I/σ(I)> | 17.3 | |
Completeness [%] | 98.1 | 99.7 |
Redundancy | 5.8 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 20 * | Imidazole, MgCl2, IPTG, DMSO, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | imidazole | 100 (mM) | |
2 | 1 | reservoir | 50 (mM) | ||
3 | 1 | reservoir | IPTG | 5 (mM) | |
4 | 1 | reservoir | DMSO | 5 (%(v/v)) |