1HXF

HUMAN THROMBIN COMPLEX WITH HIRUDIN VARIANT

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1996-02-20
Detector	SIEMENS
Spacegroup name	C 1 2 1
Unit cell lengths	70.660, 72.470, 73.090
Unit cell angles	90.00, 100.75, 90.00

Refinement procedure

Resolution	7.000 - 2.300 *
R-factor	0.151
RMSD bond length	0.014
RMSD bond angle	0.049
Data reduction software	XENGEN
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Rmerge	0.048
Total number of observations	44309 *
Number of reflections	14047 *
Completeness [%]	91.0 *
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris	0.1 (M)
2	1	reservoir	PEG4000	28 (%)
3	1	reservoir	sodium acetate	0.2 (M)
4	1	reservoir		0.19 (M)